odc1 (Proteintech)
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Odc1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+odc/ODC1+Antibody/pm41664946-113-6-7
Average 94 stars, based on 41 article reviews
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Incubation:Article Title: Targeting RNA editing of antizyme inhibitor 1 : A potential oligonucleotide-based antisense therapy for cancer Article Snippet: Protein lysates were prepared with Radioimmunoprecipitation assay (RIPA) buffer (Sigma) supplemented with 1 cOmplete EDTA-free Protease Inhibitor Cocktail (Roche) and quantified by Bradford assay (Bio-Rad). .. Protein lysates were then separated by 8%–10% SDSPAGE, followed by incubation with primary antibodies (1:1,000 dilution) overnight at 4 C and incubation with secondary antibodies (1:10,000 dilution) at room temperature for 1 h. Primary antibodies used are anti-ADAR1 (Abcam, ab88574), anti-AZIN1 (Proteintech, 11548-1-AP), anti-b-actin (1:10,000; Santa Cruz Biotechnology, sc47778), anti-Flag-horseradish peroxidase (1:10,000; Sigma-Aldrich, A8592), Software:Article Title: Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes. Article Snippet: .. For b-cell mass, pancreata were immunostained using rabbit anti-insulin (1:1000; 28728-1-AP, ProteinTech), Immpress reagent kit peroxidase conjugated anti-rabbit Ig (Vector), and DAB peroxidase substrate kit (Vector). b-cell mass was calculated by measuring insulin positive area and pancreas area using CV-X software on a Keyence fluorescent microscope system (Keyence) as described previously.48 For ODC quantification pancreata were stained for immunofluorescence using the following antibodies: Microscopy:Article Title: Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes. Article Snippet: .. For b-cell mass, pancreata were immunostained using rabbit anti-insulin (1:1000; 28728-1-AP, ProteinTech), Immpress reagent kit peroxidase conjugated anti-rabbit Ig (Vector), and DAB peroxidase substrate kit (Vector). b-cell mass was calculated by measuring insulin positive area and pancreas area using CV-X software on a Keyence fluorescent microscope system (Keyence) as described previously.48 For ODC quantification pancreata were stained for immunofluorescence using the following antibodies: Staining:Article Title: Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes. Article Snippet: .. For b-cell mass, pancreata were immunostained using rabbit anti-insulin (1:1000; 28728-1-AP, ProteinTech), Immpress reagent kit peroxidase conjugated anti-rabbit Ig (Vector), and DAB peroxidase substrate kit (Vector). b-cell mass was calculated by measuring insulin positive area and pancreas area using CV-X software on a Keyence fluorescent microscope system (Keyence) as described previously.48 For ODC quantification pancreata were stained for immunofluorescence using the following antibodies: Article Title: Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes Article Snippet: For β-cell mass, pancreata were immunostained using rabbit anti-insulin (1:1000; 28728-1-AP, ProteinTech), Immpress reagent kit peroxidase conjugated anti-rabbit Ig (Vector), and DAB peroxidase substrate kit (Vector). β-cell mass was calculated by measuring insulin positive area and pancreas area using CV-X software on a Keyence fluorescent microscope system (Keyence) as described previously. .. For ODC quantification pancreata were stained for immunofluorescence using the following antibodies: Immunofluorescence:Article Title: Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes. Article Snippet: .. For b-cell mass, pancreata were immunostained using rabbit anti-insulin (1:1000; 28728-1-AP, ProteinTech), Immpress reagent kit peroxidase conjugated anti-rabbit Ig (Vector), and DAB peroxidase substrate kit (Vector). b-cell mass was calculated by measuring insulin positive area and pancreas area using CV-X software on a Keyence fluorescent microscope system (Keyence) as described previously.48 For ODC quantification pancreata were stained for immunofluorescence using the following antibodies: Article Title: Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes Article Snippet: For β-cell mass, pancreata were immunostained using rabbit anti-insulin (1:1000; 28728-1-AP, ProteinTech), Immpress reagent kit peroxidase conjugated anti-rabbit Ig (Vector), and DAB peroxidase substrate kit (Vector). β-cell mass was calculated by measuring insulin positive area and pancreas area using CV-X software on a Keyence fluorescent microscope system (Keyence) as described previously. .. For ODC quantification pancreata were stained for immunofluorescence using the following antibodies: other:Article Title: Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes Article Snippet: Anti-ODC , Article Title: Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes. Article Snippet: Alexa Fluor antibodies were used as secondary antibodies (Invitrogen). |
